Migrasi Fibroblas pada Penderita COPD
COPD adalah penyakit dengan progresi lambat ditandai empisema, fibrosis saluran napas kecil & saluran napas obstruktif
Migration of Human COPD Fibroblasts
Kirsi Vuorinen,1 Marjukka Myll�rniemi,1 Vuokko Kinnula.1 1Department of Medicine, University of Helsinki, Helsinki, Finland
Chronic obstructive pulmonary disease (COPD) is a slowly progressive disease characterized by emphysema, small airway fibrosis and airway obstruction. Various cytokines and growth factors have been implicated in the pathophysiology of COPD and in the structural changes seen in lung parenchyma. Platelet-derived growth factor (PDGF) signaling is known to promote extracellular matrix deposition, as well as mesenchymal cell proliferation and survival during fibrogenesis. Using primary human pulmonary fibroblasts from a patient with COPD, we examined the influence of several cytokines on cellular migration.
Primary cells were isolated from the explanted lung after lung transplantation. The myofibroblast phenotype (α-smooth muscle actin) of the cells was established after 2 passages using immunohistochemistry. Cellular migration was quantified using two-chamber culture plates with polycarbonate membranes (pore size 8 μm) coated with 1μg/ml fibronectin. Cells were exposed to known profibrotic cytokines (EGF, PDGF-AB, PDGF-BB, TGF-β) and the migrated cells were counted.
The results showed that PDGF-AB and PDGF-BB were the strongest stimulators of COPD fibroblast migration with mean cell counts of 1029 � 170 and 723 � 53 migrated cells, respectively, compared to 211 � 136 control cells, stimulated with 10% serum. On contrary to normal human pulmonary fibroblasts, EGF seemed to have no effect on the migration of cells from a patient with COPD (150 � 45). These results indicate that PDGF-A may play a role in COPD as well as in pulmonary fibrosis in recruiting myofibroblasts and stimulating their migration.
Session Info: Free Access Electronic Poster Session: Airway cell biology I
Eur Respir Journal�E4294
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